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法國Biocytex 
法國BIOCYTEX公司建立于20世紀(jì)90年代初，多年來一直致力于流式細(xì)胞術(shù)檢測試劑的研究，并已取得了突破性進(jìn)展，目前已有成熟的產(chǎn)品面市，其產(chǎn)品的特點(diǎn)包括：血小板分析使用全血樣本進(jìn)行檢測；樣本處理過程中不會(huì)引起血小板激活；全程免洗；樣本用量少；操作簡便、快捷；采用獨(dú)特的定量技術(shù)，可以準(zhǔn)確定量細(xì)胞表面特定蛋白分子或受體的數(shù)量。
法國BIOCYTEX公司研發(fā)的一系列關(guān)于血小板方面的定量流式全血細(xì)胞檢測試劑盒，對(duì)監(jiān)控抗血小板藥物和協(xié)助診斷血小板錯(cuò)亂疾病起到了積極作用。而同樣的定量檢測技術(shù)也應(yīng)用于白細(xì)胞和紅細(xì)胞受體分析，這些試劑盒的生產(chǎn)對(duì)陣發(fā)性睡眠癥（PNH）提供診斷標(biāo)準(zhǔn)。
目前公司產(chǎn)品主要由一下幾類組成：應(yīng)用于流式細(xì)胞術(shù)17類（其中血小板10種），用于分子生物學(xué)5類，ELISA試劑盒3類（包括檢測重組細(xì)胞因子CD146），抗體共計(jì)56種，其中CD抗體共有49種。
   

Since the foundation of Biocytex in the early 90's in the close proximity of the University and Hospital Complex of La Timone / La Conception in Marseille (France), the need of standardization in flow cytometry analysis still remains a topic of concern. Inter-laboratory reproducibility, day to day reproducibility and positivity and/or negativity threshold determination are a real need that can be addressed by the use of quantitative flow cytometry application kits. This is the expertise offered by Biocytex. We spent 7 years developing and tuning our quantitative flow cytometry technology. After the success of our first generation of products with the QIFIKIT, we introduced our PLATELET kits, a comprehensive line of no wash whole blood flow cytometry kits for the monitoring of antiplatelet drugs (PLATELET VASP/P2Y12) as well as the diagnosis of platelet disorders (PLATELET Gp/Receptors).
The same quantitative technology was applied to the development of kits for leukocyte and red cell receptor analysis (CELLQUANT / REDQUANT kits). A major application is offered for the standardized diagnosis of Paroxysmal Nocturnal Hemoglobinuria (PNH).
In the past two years, the aim of BioCytex was to increase its range of products in the field of Thrombosis and Haemostasis.

Quantitative flow cytometry technology

On a small sample volume containing few cells and heterogeneous cell populations, flow cytometry allows to analyze with high sensitivity the cell size, its contents and the intensity of these stained cells.
Although in current practice of immunophenotyping flow cytometers are mainly used to differentiate positive and negative staining of cells, BioCytex uses these same instruments to quantitate molecules bound on cells, receptors, ligands or drugs as well as intracellular targets.

How does quantitative flow cytometry work ?

Quantitative flow cytometry is achieved by comparing fluorescence intensity and a calibration standard.
BioCytex uses a calibrator made of a mix of bead populations . These calibration beads are coated with known and increasing numbers of immunoglobulins (IgG) bound to their surface thus mimicking the binding of specific monoclonal antibodies to the biological sample cells.
Then, this calibrator and the target stained cells are incubated with polyclonal antibodies labeled with FITC fluorochrome. The beads bear specific number of sites and will allow to draw a calibration curve relating mean fluorescence intensity into a number of receptors per cell. The mean fluorescence intensity (MFI) of the tested sample is reported on the calibration curve to determine the number of molecules expressed per cell. Non specific staining is evaluated using an irrelevant antibody (negative isotypic control).